منابع مشابه
Expressed Sequence Tags
Now a days, genome analysis has employed a rapid analysis tool known as expressed sequence tags. In 1983, SD Putney for the first time demonstrated the use of cDNA in identification of genome. The term expressed sequence tags (ESTs) was coined by Anthony Kerlavage at the Institute for Genomic Research. In 1991, Mark Adams used EST in relation to gene discovery and Human Genome project. cDNA or ...
متن کاملProtein identification with sequence tags
Genome sequences are available for increasing numbers of organisms. The proteomes (protein complement expressed by the genome) of some such organisms are being studied with two-dimensional gel electrophoresis, but the identification of thousands of proteins on two-dimensional gels remains a challenge. Recent progress with mass spectrometric and traditional sequencing methods has increased the s...
متن کاملNot All Sequence Tags Are Created Equal: Designing and Validating Sequence Identification Tags Robust to Indels
Ligating adapters with unique synthetic oligonucleotide sequences (sequence tags) onto individual DNA samples before massively parallel sequencing is a popular and efficient way to obtain sequence data from many individual samples. Tag sequences should be numerous and sufficiently different to ensure sequencing, replication, and oligonucleotide synthesis errors do not cause tags to be unrecover...
متن کاملProtEST: protein multiple sequence alignments from expressed sequence tags
MOTIVATION An automatic sequence searching method (ProtEST) is described which constructs multiple protein sequence alignments from protein sequences and translated expressed sequence tags (ESTs). ProtEST is more effective than a simple TBLASTN search of the query against the EST database, as the sequences are automatically clustered, assembled, made non-redundant, checked for sequence errors, ...
متن کاملExpressed sequence tags: analysis and annotation.
Expressed sequence tags (ESTs) present a special set of problems for bioinformatic analysis. They are partial and error-prone, and large datasets can have significant internal redundancy. To facilitate analysis of small EST datasets from in-house projects, we present an integrated "pipeline" of tools that take EST data from sequence trace to database submission. These tools also can be used to ...
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ژورنال
عنوان ژورنال: Nature
سال: 1992
ISSN: 0028-0836,1476-4687
DOI: 10.1038/357367a0